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Invitrogen
GLP-1 Monoclonal Antibody (03)
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Product Details
ABS 046-03-02
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mammal,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
03
Immunogen
Synthetic GLP-1(9-37) adsorbed onto aluminum hydroxide gel.
Conjugate
Form
Liquid
Concentration
1.03 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536223
Product Specific Information
Specificity: ABS 046-03-02 binds GLP-1 (1-37), GLP-1 (7-37) and GLP-1 (9-37). This antibody shows 0-16% cross-Specificity with C-terminally amidated GLP-1 in various circumstances.
Reactivity: ABS 046-03-02 binds free GLP-1 (1-37), GLP-1 (7-37) and GLP-1 (9-37) in solution. Two different ELISA sandwich setups can be used for measuring non-amidated GLP-1 forms: The first uses ABS 046-03 as a capture antibody with biotinylated detection antibody ABS 033-10-02. This shows ~5% cross-reactivity with C-terminally amidated GLP-1. The second combination uses HYB 147-12 as a capture antibody with biotinylated detection antibody ABS 046-03-02, resulting in ~16% cross-reactivity with C-terminally amidated GLP-1. Results show detection limits of 44 pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Target Information
GCG is also known as GLP1, or Glucagon. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis.It is mapped to 2q36-2q37. GLP1, also known as 7-37 for the codons of the preproglucagon molecule which encode it, renders pancreatic beta-cells 'glucose-competent' and may be useful in the treatment of noninsulin-dependent diabetes mellitus. Also, GLP1 is a potent insulin secretagogue.It plays a major role in the enteroinsular axis, accounting, for example, for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glicentin-related polypeptide; GLP1/2; glucagon; Glucagon like peptide 1; glucagon-like peptide 1; glucagon-like peptide 2; glucagon-like peptide-1; MGC138331; OXY; preproglucagon; Pro-glucagon
Gene Aliases: GCG; GLP-1; GLP1; GLP2; Glu; GRPP; PPG
UniProt ID: (Human) P01275, (Mouse) P55095
Entrez Gene ID: (Human) 2641, (Mouse) 14526, (Rat) 24952
regulation of carbohydrate metabolic process
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
protein kinase A signaling
positive regulation of peptidyl-threonine phosphorylation
regulation of lipid metabolic process
protein metabolic process
positive regulation of cAMP biosynthetic process
positive regulation of protein binding
negative regulation of appetite
positive regulation of peptidyl-serine phosphorylation
positive regulation of insulin secretion involved in cellular response to glucose stimulus
positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter
response to starvation
negative regulation of apoptotic process
positive regulation of protein kinase activity
regulation of insulin secretion
positive regulation of histone H3-K4 methylation
positive regulation of ERK1 and ERK2 cascade
positive regulation of calcium ion import
negative regulation of execution phase of apoptosis
negative regulation of intrinsic apoptotic signaling pathway
signal transduction
G-protein coupled receptor signaling pathway
feeding behavior
cell proliferation
cellular response to glucagon stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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